imagequant software version 5·2 Search Results


imagequant version 5 2 software  (Molecular Dynamics Inc)
86
Molecular Dynamics Inc imagequant version 5 2 software
Imagequant Version 5 2 Software, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagequant+software+version+5%C2%B72/imagequant+software/10__1161_slash_01__atv__0000189159__96900__d9-170-7-11
Average 86 stars, based on 1 article reviews
imagequant version 5 2 software - by Bioz Stars, 2026-10
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imagequant 5 2 software  (Amersham Life Sciences Inc)
86
Amersham Life Sciences Inc imagequant 5 2 software
Imagequant 5 2 Software, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagequant+software+version+5%C2%B72/imagequant/pm20658525-66-5-8
Average 86 stars, based on 1 article reviews
imagequant 5 2 software - by Bioz Stars, 2026-10
86/100 stars
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imagequant 5 2 1 software  (Bio-Rad)
96
Bio-Rad imagequant 5 2 1 software
Imagequant 5 2 1 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagequant+software+version+5%C2%B72/ChromLab+Software/pmc05442622-322-0-3
Average 96 stars, based on 1 article reviews
imagequant 5 2 1 software - by Bioz Stars, 2026-10
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odyssey infrared imaging system  (LI-COR)
99
LI-COR odyssey infrared imaging system
Detection of Alexa680-labeled SUMO transfer from E1 to E2 (Ubc9). E1•SUMO thioester is formed initially as described in Basic Protocol 4. Then the reaction is quenched with EDTA, and E2 (Ubc9) was added to a final concentration of 0.2 μM. Aliquots of the reaction mixture were withdrawn at 0, 15, 30, 45 and 60 s after E2(Ubc9) addition and mixed with 2X SDS sample buffer (containing no DTT) with 8 M urea. Steps 8 and 9 should be performed at 4 ºC. Formation of the E2•SUMO complex was resolved by gel electrophoresis and visualized with the <t>Odyssey</t> <t>infrared</t> <t>imaging</t> <t>system.</t> Quantification of the transfer assays was carried out using ImageQuant 5.2 software.
Odyssey Infrared Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagequant+software+version+5%C2%B72/Odyssey+Imaging+System/pmc03477621-538-116-120
Average 99 stars, based on 1 article reviews
odyssey infrared imaging system - by Bioz Stars, 2026-10
99/100 stars
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v 2006 software  (TotalLab Ltd)
97
TotalLab Ltd v 2006 software
Detection of Alexa680-labeled SUMO transfer from E1 to E2 (Ubc9). E1•SUMO thioester is formed initially as described in Basic Protocol 4. Then the reaction is quenched with EDTA, and E2 (Ubc9) was added to a final concentration of 0.2 μM. Aliquots of the reaction mixture were withdrawn at 0, 15, 30, 45 and 60 s after E2(Ubc9) addition and mixed with 2X SDS sample buffer (containing no DTT) with 8 M urea. Steps 8 and 9 should be performed at 4 ºC. Formation of the E2•SUMO complex was resolved by gel electrophoresis and visualized with the <t>Odyssey</t> <t>infrared</t> <t>imaging</t> <t>system.</t> Quantification of the transfer assays was carried out using ImageQuant 5.2 software.
V 2006 Software, supplied by TotalLab Ltd, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagequant+software+version+5%C2%B72/Phoretix+1D/pm22285271-80-21-18
Average 97 stars, based on 1 article reviews
v 2006 software - by Bioz Stars, 2026-10
97/100 stars
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imagequant 5 2 software  (Amersham Pharmacia Biotech Ltd)
86
Amersham Pharmacia Biotech Ltd imagequant 5 2 software
Detection of Alexa680-labeled SUMO transfer from E1 to E2 (Ubc9). E1•SUMO thioester is formed initially as described in Basic Protocol 4. Then the reaction is quenched with EDTA, and E2 (Ubc9) was added to a final concentration of 0.2 μM. Aliquots of the reaction mixture were withdrawn at 0, 15, 30, 45 and 60 s after E2(Ubc9) addition and mixed with 2X SDS sample buffer (containing no DTT) with 8 M urea. Steps 8 and 9 should be performed at 4 ºC. Formation of the E2•SUMO complex was resolved by gel electrophoresis and visualized with the <t>Odyssey</t> <t>infrared</t> <t>imaging</t> <t>system.</t> Quantification of the transfer assays was carried out using ImageQuant 5.2 software.
Imagequant 5 2 Software, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagequant+software+version+5%C2%B72/2+5+imagequant+software/pm15985534-89-19-22
Average 86 stars, based on 1 article reviews
imagequant 5 2 software - by Bioz Stars, 2026-10
86/100 stars
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Image Search Results


Detection of Alexa680-labeled SUMO transfer from E1 to E2 (Ubc9). E1•SUMO thioester is formed initially as described in Basic Protocol 4. Then the reaction is quenched with EDTA, and E2 (Ubc9) was added to a final concentration of 0.2 μM. Aliquots of the reaction mixture were withdrawn at 0, 15, 30, 45 and 60 s after E2(Ubc9) addition and mixed with 2X SDS sample buffer (containing no DTT) with 8 M urea. Steps 8 and 9 should be performed at 4 ºC. Formation of the E2•SUMO complex was resolved by gel electrophoresis and visualized with the Odyssey infrared imaging system. Quantification of the transfer assays was carried out using ImageQuant 5.2 software.

Journal: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]

Article Title: Biochemical Analysis of Protein SUMOylation

doi: 10.1002/0471142727.mb1029s99

Figure Lengend Snippet: Detection of Alexa680-labeled SUMO transfer from E1 to E2 (Ubc9). E1•SUMO thioester is formed initially as described in Basic Protocol 4. Then the reaction is quenched with EDTA, and E2 (Ubc9) was added to a final concentration of 0.2 μM. Aliquots of the reaction mixture were withdrawn at 0, 15, 30, 45 and 60 s after E2(Ubc9) addition and mixed with 2X SDS sample buffer (containing no DTT) with 8 M urea. Steps 8 and 9 should be performed at 4 ºC. Formation of the E2•SUMO complex was resolved by gel electrophoresis and visualized with the Odyssey infrared imaging system. Quantification of the transfer assays was carried out using ImageQuant 5.2 software.

Article Snippet: Materials 10X ATP regeneration system (see recipe) 100 μM SUMO (see Support Protocol) 1 μM E1 enzyme (see Support Protocol) 250, 400, and 625 nM Ubc9 (see Support Protocol) 100 μM RanGAP1 (see Support Protocol) 50 mM Tris-HCl (pH 7.5) Milli-Q-purified water 2XSDS sample buffer with DTT 4–12% Bis-Tris gradient gel (Invitrogen) NuPAGE MOPS SDS running buffer Mouse monoclonal primary antibody against SUMO-1 (Abgent) IRDye 680-labeled secondary antibody (Li-Cor Biosciences) Phosphate Buffered Saline (PBS) (see recipe) Phosphate Buffered Saline Tween-20 (PBST) (see recipe) Blocking buffer (Odyssey, Li-Cor Biosciences) 37 ºC heat block Ice bucket Micropipette SDS-PAGE apparatus (Invitrogen) Rocking shaker (Reliable Scientific, Inc.) 0.2 μM PVDF trans-blot turbo membrane (Bio-Rad) Trans-blot turbo transfer system apparatus (Bio-Rad) Odyssey infrared imaging system (Li-Cor Biosciences) ImageQuant 5.2 software (GE Life Sciences) Set up of the RanGAP1-SUMO isopeptide complex formation assays Equilibrate heat block to 37 ºC.

Techniques: Labeling, Concentration Assay, Nucleic Acid Electrophoresis, Imaging, Software